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Tuesday, May 12, 2020 | History

2 edition of Leukocyte adhesion from flowing whole blood in vitro found in the catalog.

Leukocyte adhesion from flowing whole blood in vitro

Katherine Bernice Abbitt

Leukocyte adhesion from flowing whole blood in vitro

influence of haematological and rheological parameters

by Katherine Bernice Abbitt

  • 193 Want to read
  • 31 Currently reading

Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Birmingham, Department of Physiology, Division of Medical Sciences, Faculty of Medicine and Dentistry.

Statementby Katherine Bernice Abbitt.
The Physical Object
Pagination293 p. :
Number of Pages293
ID Numbers
Open LibraryOL19281642M

Leukocyte adhesion has been the subject of intense investigation during the past decades and this has resulted in the concept of the leukocyte adhesion cascade in the early s 2, 3. Leukocyte adhesion is initiated by the selectin-mediated capture of leukocytes to the endothelium, causing the cells to roll over the vascular : Tanja Vajen, Alexandra C. A. Heinzmann, Annemiek Dickhout, Zhen Zhao, Magdolna Nagy, Johan W. M. Hee. Cell adhesion and leukocyte adhesion defects. In Ochs HD, Puck JM, Smith CIE, Primary Immunodeficiency diseases. A molecular and genetic approach. Sec Ed Oxford 8. Etzioni A, Frydman M, Pollack S, Avidor I, Phillips ML, Paulson JC, Gershoni-Baruch R. Brief report: recurrent severe infections caused by a novel leukocyte adhesion deficiency.

Start studying What is leukocyte adhesion deficiency?. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Leukocyte Adhesion Deficiency or L.A.D. is a very rare genetic disorder that affects the body's immune system. L.A.D. occurs when a patient's white blood cells or .

for studying leukocyte adhesion in the context of whole blood and without blood cell isolation. The microscopic observation of leukocyte rolling in different in vivo and in vitro settings has significantly contributed to our understanding of the molecular mechanisms responsible for the stepwise extravasation of leukocytes into inflamed tissues. The Leukocyte Adhesion Deficiency Panel measures the receptors CD11b, CD15, and CD18 on neutrophils. The percentage of patient neutrophils bearing these receptors is reported. Although these receptors are normally found on neutrophils; values outside of the reference interval may correlate with abnormal neutrophil function.


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Leukocyte adhesion from flowing whole blood in vitro by Katherine Bernice Abbitt Download PDF EPUB FB2

Leukocyte adhesion deficiency (LAD) is a rare primary immunodeficiency. The clinical picture is characterized by marked leukocytosis and localized bacterial infections that are difficult to detect until they have progressed to an extensive level secondary to.

Leukocyte extravasation (also commonly known as Leukocyte adhesion cascade) is the movement of leukocytes out of the circulatory system and towards the site of tissue damage or infection. This process forms part of the innate immune response, involving the recruitment of non-specific leukocytes.

Monocytes also use this process in the absence of infection or tissue damage during their. The flow chamber is a versatile tool for examining adhesive interactions, as it can be used to study a variety of adhesive substrates, ranging from monolayers of primary cells to isolated adhesion molecules, and a variety of adhesive particles, ranging from leukocytes in Cited by: 6.

Platelet-leukocyte aggregates can also be found in whole blood (10, 11). Mutually activating interactions can also occur between leukocytes and platelets through released substances, and these interactions are promoted by adhesion between the cells.

Platelet-leukocyte adhesion may thus be influential in the development of inflammation or of Cited by: 4. Hidalgo A, Ma S, Peired AJ, et al.

Insights into leukocyte adhesion deficiency type 2 from a novel mutation in the GDP-fucose transporter gene. Blood ; Bunting M, Harris ES, McIntyre TM, Prescott SM, Zimmerman GA.

Leukocyte adhesion deficiency syndromes: adhesion and tethering defects involving B2 integrins and selectin ligands. In flowing blood, leukocytes roll on adherent activated platelets, mainly through the interaction of platelet P-selectin with its major leukocyte ligand, PSGL 45 This initial rolling of leukocytes on platelet P-selectin is followed by their firm adhesion and subsequent migration—processes that depend on the leukocyte integrin Mac-1 (αMβ2.

Leukocyte recruitment to a site of endothelial injury. When injured, the endothelium expresses an increase in cell adhesion molecules (CAMs). (A) Leukocytes circulate within the blood stream. Current in vitro models of the leukocyte adhesion cascade cannot be used for real-time studies of the entire leukocyte adhesion cascade, including rolling, adhesion, and migration in a single assay.

Leukocyte adhesion proceeds in a cascade-like fashion, starting with capture and followed by rolling and arrest. In all leukocytes, rapid arrest can Cited by: Leukocyte adhesion deficiency type I is by far the most common and results from a failure to express CD18, which is a subunit of integrin molecules.

Without integrins, the second step of the extravasation process called adhesion cannot happen - in other words, phagocytes can’t firmly bind to. Intravital Microscopy to Study Leukocyte Rolling In Vivo. The direct microscopic observation of flowing blood in vivo was reported for the first time by Malpighi in who found “almost an infinite number of particles” moving in capillaries of the frog lung.

Since then many modifications have enabled scientists to investigate microcirculatory phenomena including leukocyte rolling and Cited by: Type I and II leukocyte adhesion deficiency. Panel measures CD11b, CD15, and CD18 on neutrophils. Methodology. Semi-Quantitative Flow Cytometry * Performed by affiliate laboratory.

Turnaround Time. 3 days. Specimen Requirements. 5 mL (min. 1 mL) sodium heparin or lithium heparin whole blood. Specimen Stability Stable for 48 hours at 4°C. Cell Biolabs’ CytoSelect™ Leukocyte-endothelium Adhesion Assay provides a robust system for the quantitative determination of leukocyte-endothelium interactions.

The kit contains sufficient reagents for the evaluation of assays in a well plate. Figure 1. The Leukocyte-Endothelium Adhesion Cascade. Leukocyte adhesion deficiency (LAD), is a rare autosomal recessive disorder characterized by immunodeficiency resulting in recurrent infections.

LAD is currently divided into three subtypes: LAD1, LAD2, and the recently described LAD3, also known as LAD-1/ LAD3, the immune defects are supplemented by a Glanzmann thrombasthenia-like bleeding lty: Immunology.

Diagnosis of leukocyte adhesion deficiency is by detecting absence or severe deficiency of adhesive glycoproteins on the surface of WBCs using monoclonal antibodies (eg, anti-CD11, anti-CD18) and flow cytometry.

Leukocytosis detected by complete blood count is common but nonspecific. Therefore, an in vitro study was conducted to define the leukocyte removal rate of a transfusion leukocyte-depletion filter, using cell-washed and unwashed whole porcine blood.

In addition, the influence of different cell-washing protocols on the elimination rate Cited by: 3. systemic circulation, while firm adhesion of rolling leukocytes is dependent on a4/vascular cell adhesion molecule (VCAM)-1 (6) or b2/intercellular adhesion molecule (ICAM)-1 interac-tions (7).

In contrast, relatively little is known about factors that regulate various steps of leukocyte adhesion and their transmigration across TMV in vivo. Leukocyte adhesion deficiency type 1 is a disorder that causes the immune system to malfunction, resulting in a form of immunodeficiency.

Immunodeficiencies are conditions in which the immune system is not able to protect the body effectively. TRANSLATIONAL PHYSIOLOGY Whole blood and leukocyte RNA isolation for gene expression analyses Robert J. Feezor,1 Henry V. Baker,2 Michael Mindrinos,3 Doug Hayden,4 Cynthia L.

Tannahill,1 Bernard H. Brownstein,5 Adrian Fay,6 Sandra MacMillan,5 Jason Laramie,5 Wenzhong Xiao,3 Lyle L. Moldawer,1 J. Perren Cobb,5 Krzysztof Laudanski,7 Carol L. Miller-Graziano,7 Ronald V. Maier,8 Cited by: Peripheral-blood lymphocytes from the BLAD calf also expressed negligible levels of leukocyte integrins, similar to their neutrophil counterparts.

Our experimental findings in vitro correlate well with the clinical observations of decreased leukocyte trafficking and diminished host defense in leukocyte adhesion-deficient animals. Leukocyte adhesion deficiency (LAD) is an autosomal recessive disorder of neutrophil function resulting from a deficiency of the beta-2 integrin subunit of the leukocyte cell adhesion molecule.

The leukocyte cell adhesion molecule is present on the surface of peripheral blood mononuclear leukocytes and granulocytes and mediates cell-cell and.Blood ; These authors use low concentration ADP and epinephrine to detect hyperaggregation. These authors use low concentration ADP and epinephrine to detect hyperaggregation.

You may want to follow their model, however if you are using the more up-to-date whole blood aggregometry, epinephrine does not work.

Characteristics of leucocyte adhesion directly observed in flowing whole blood in vitro. Br J Haematol. ;– 15 [[15]] Cornish RJ. Flow in a pipe of rectangular cross-section. Proc Roy Soc Lond A. ;– 16 [[16]] Andrews RK, Gardiner EE, Shen Y, Whisstock JC, Berndt MC.

Glycoprotein Ib-IX-V. Int J Biochem Cell Biol Cited by: 4.